Table 1 Primer sequences for quantitative RT-PCR.
Gene | Gene bank no. | Direction | Primer sequence |
|---|---|---|---|
Human PPIA | NM_021130 | F | TGCCATCGCCAAGGAGTAG |
R | TGCACAGACGGTCACTCAAA | ||
Human IL6 | NM_000600 | F | GGTACATCCTCGACGGCATCT |
R | GTGCCTCTTTGCTGCTTTCAC | ||
Human P16 | NM_000077 | F | CTACTGAGGAGCCAGCGTCT |
R | CTGCCCATCATCATGACCT | ||
Human P18 | NM_001262 | F | TGGGTCTTCCGCAAGAACTC |
R | TGGCAGCCAAGTGCAAGGGC | ||
Human P21 | NM_000389 | F | ACAGCAGAGGAAGACCATGTGGACC |
R | CGTTTTCGACCCTGAGAGTCTCCAG | ||
Human C-MYC | NM_002467 | F | AAAGGCCCCCAAGGTAGTTA |
R | GCACAAGAGTTCCGTAGCTG | ||
Human KLF4 | NM_001314052 | F | GAACTGACCAGGCACTACCG |
R | TTCTGGCAGTGTGGGTCATA | ||
Human NANOG | NM_024865 | F | TGGGATTTACAGGCGTGAGC |
R | AAGCAAAGCCTCCCAATCCC | ||
Human OCT4 | NM_002701 | F | AGCCCTCATTTCACCAGGCC |
R | TGGGACTCCTCCGGGTTTTG | ||
Human SOX2 | NM_003106 | F | AAATGGGAGGGGTGCAAAAGAGGAG |
R | CAGCTGTCATTTGCTGTGGGTGATG | ||
Human LHR | NM_000233 | F | ACCTCCCTGTCAAAGTGATCC |
R | AGGTTGTCAAAGGCATTAGCTTC | ||
Mouse HPRT | NM_013556 | F | GCCTAAGATGAGCGCAAGTTG |
R | TACTAGGCAGATGGCCACAGG | ||
Mouse C-MYC | NM_010849 | F | CGCACACACAACGTCTTGGA |
R | AGGATGTAGGCGGTGGCTTT | ||
Mouse KLF4 | NM_010637 | F | GGTGCAGCTTGCAGCAGTAA |
R | AAAGTCTAGGTCCAGGAGGT | ||
Mouse OCT4 | NM_013633 | F | GCATTCAAACTGAGGCACCA |
R | AGCTTCTTTCCCCATCCCA | ||
Mouse SOX2 | NM_011443 | F | GAAGCGTGTACTTATCCTTCTTCAT |
R | GAGTGGAAACTTTTGTCCGAGA | ||
Mouse IL6 | NM_031168 | F | TAGTCCTTCCTACCCCAATTTCC |
R | TTGGTCCTTAGCCACTCCTTC | ||
Mouse P16 | NM_001040654 | F | TTGAGCAGAAGAGCTGCTACGT |
R | CGTACCCCGATTCAGGTGAT | ||
Mouse P18 | NM_001301368 | F | GAACCATAAGGGGGACACCG |
R | CCATTTGCCTCCATCAGGCT | ||
Mouse P21 | NM_007669 | F | GGTTCCTTGCCACTTCTT |
R | GAGTCGGGATATTACGGTTG |
