Fig. 1: Illustration from Burguillos et al. 2015 [31]: Gal-3 acts as a ligand to TLR4.
From: Galectin-3, a rising star in modulating microglia activation under conditions of neurodegeneration
Left panel: Colocalisation of Gal-3 and TLR4 in BV2 cells after 1 h exposure with sGal-3 protein. Right panel: Microscale thermophoresis was used to analyse the direct binding of TLR4 to Gal-3, Gal-3 CRD, Gal-3 R186S, and Gal-3 in the presence of inhibitory lactose (40 mM). Whereas the concentration of fluorescently labelled TLR4 was kept constant, the non-labelled proteins were titrated (x axis), and the minimal and maximal Fnorm values of the unbound and bound state of TLR4, respectively, were used to calculate the percent of TLR4 bound to Gal-3 (y axis).
