Fig. 5: Semaphorin 5A reduces ferroptosis of SFs by GPX4. | Cell Death & Disease

Fig. 5: Semaphorin 5A reduces ferroptosis of SFs by GPX4.

From: Semaphorin 5A suppresses ferroptosis through activation of PI3K-AKT-mTOR signaling in rheumatoid arthritis

Fig. 5: Semaphorin 5A reduces ferroptosis of SFs by GPX4.The alternative text for this image may have been generated using AI.

A mRNA levels of GPX4 in SFs detected by qPCR (n = 3) after stimulation with different concentrations of Semaphorin 5A. B Protein abundances of GPX4 in SFs detected by western blotting (n = 3) after stimulation with Semaphorin 5A (1 μg/mL). C SF mitochondrial morphology observed by transmission electron microscopy (n = 3). D Intracellular MDA levels in SFs detected by a Lipid Oxidation (MDA) Assay Kit (n = 3). E Fluorescence intensity of C11-BODIPY 581/591 in SFs detected by flow cytometry (n = 3). F ROS levels in SFs detected by flow cytometry (n = 3). G Intracellular MDA levels in SFs detected by a Lipid Oxidation (MDA) Assay Kit after treatment with pictilisib (10 μM), MK-2206 (5 μM), temsirolimus (100 nM), and Semaphorin 5A (1 μg/mL) (n = 3). H–I mRNA levels and protein abundances of GPX4 in SFs detected by qPCR (H, n = 3) and western blotting (I, n = 3) after treatment with pictilisib (10 μM), MK-2206 (5 μM), temsirolimus (100 nM), and Semaphorin 5A (1 μg/mL). *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant. Values of P < 0.05 were considered significant. SFs synovial fibroblasts.

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