Fig. 3: CKD serum induced endothelial dysfunction and ANRIL upregulation.
From: LncRNA ANRIL mediates endothelial dysfunction through BDNF downregulation in chronic kidney disease
A Protein and mRNA levels of vWF, eNOS, VCAM-1 in HUVECs exposed to CKD serum. B Protein levels of BDNF in HUVECs exposed to CKD serum. C Representative immunofluorescence images of VCAM-1 in HUVECs exposed to CKD serum. Scale bars = 50 μm. D Mitosox Red staining showed the mitochondrial ROS level of endothelial cells. Scale bars = 50 μm. E Mitotracker Red probe was used to label mitochondria. Scale bars = 20 μm. F Representative immunofluorescence images of Mfn2 in HUVECs exposed to CKD serum. Scale bars = 50μm. G Expression of Mfn2, Drp1 in HUVECs were detected by Western blot. H ANRIL expression in HUVECs were detected by realtime PCR. I FISH assays was used to detect the expression and subcellular location of ANRIL in endothelial cells. Scale bars = 25 μm. Data were shown as mean ± SD (n = 3). (Student’s t test, *P < 0.05 versus control).
