Fig. 4: Uremia toxin induced ANRIL expression and ANRIL mediated endothelial dysfunction.
From: LncRNA ANRIL mediates endothelial dysfunction through BDNF downregulation in chronic kidney disease
A–D ANRIL expression in endothelial cells stimulated with (A) IS, (B) HA, (C) IAA, (D) Hcy at the concentrations indicated for 48 h. E Protein levels of vWF, eNOS, VCAM-1 in HUVECs exposed to IS at different doses. F Protein levels of Mfn2, Drp1 in HUVECs exposed to IS at different doses. G Protein and mRNA levels of vWF, eNOS, VCAM-1 in HUVECs infected with Sh-ANRIL ShRNA under the IS stimulation. H Protein levels of Mfn2, Drp1 in HUVECs. I Protein and mRNA levels of vWF, eNOS, VCAM-1 in HUVECs with ANRIL overexpression or inhibition. J Expression of Mfn2, Drp1 in HUVECs were detected by Western blot. Data were shown as mean ± SD (n = 3). (one-way ANOVA and the Tukey post-test. *P < 0.05 versus respective control, #P < 0.05 versus IS or ANRIL overexpression). Sh-ANR: Sh-ANRIL infection, Scr: Scramble, ANR: ANRIL overexpression.
