Fig. 7: The HOXA10-AS transcript serves as a modular scaffold for TP63 RNA-processing.

A An RNA-IP assay was performed with control IgG and UPF1-specific antibodies. The precipitated RNA was analyzed by RT-qPCR with the specific primers (n = 3). B Control and UPF1-specific siRNA were delivered into cells, and the expression of the indicated protein and mRNA of transfected cells was measured by Western blot and RT-qPCR (n = 3), respectively. GAPDH expression served as the loading control. C UPF1 knockdown cells were collected and subjected to a fractionation assay. TP63 gene expression in fractions was detected by RT-qPCR (n = 5). D An RNA-IP assay of the indicated cells was performed with UPF1-specific antibody, and the precipitated RNA was subjected to RT-qPCR assay (n = 4). E MS2-based RNA-IP assay of the indicated transfected cells was performed, and the precipitated RNA was subjected to RT-qPCR assay with specific primers (n = 3). F UPF1 siRNA was transfected into knockdown cells, and TP63 expression of transfected cells was detected by RT-qPCR experiments (n = 5). G Schematic representation of HOXA10-AS lncRNA-mediated TP63 gene regulation is shown.