Fig. 6: TFEB-dependent MSCs therapy attenuated Beclin1 ubiquitination of ataxin-3 protein and the toxic effects of mutant proteins in SCA3 neurons. | Cell Death & Disease

Fig. 6: TFEB-dependent MSCs therapy attenuated Beclin1 ubiquitination of ataxin-3 protein and the toxic effects of mutant proteins in SCA3 neurons.

From: Transcription factor EB-mediated mesenchymal stem cell therapy induces autophagy and alleviates spinocerebellar ataxia type 3 defects in neuronal cells model

Fig. 6: TFEB-dependent MSCs therapy attenuated Beclin1 ubiquitination of ataxin-3 protein and the toxic effects of mutant proteins in SCA3 neurons.

A A diagram that comes from www.kegg.jp/pathway. B The effects of GW4869 and 3-MA on the interaction of Beclin1 and ataxin-3 using Co-IP. C The effects of shTFEB on the interaction of Beclin1 and ataxin-3 through Co-IP. D The effects of TFEB on the interaction of Beclin1 and ataxin-3 by Co-IP. E The effects of shTFEB and TFEB on the interaction of Beclin1 and ataxin-3 by immunofluorescence (scale bar: 50 μm). F The effects of shTFEB and TFEB on the neuron-specific marker MAP2 after MSCs by immunofluorescence (scale bar: 20 μm). G The effects of shTFEB and TFEB on neuron survival and proliferation. H The effects of shTFEB, TFEB, 3-MA, and GW4869 on the synaptic protein PSD95 after MSCs (scale bar: 50 μm). I Schematic diagram of the proposed mechanism. *p < 0.05, **p < 0.01, ***p < 0.001 vs Control group, #p < 0.05, ##p < 0.01, ###p < 0.001.

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