Fig. 2: PCDH1 promotes the growth, metastasis and side population phenotype acquisition of PDAC cells in vitro. | Cell Death & Disease

Fig. 2: PCDH1 promotes the growth, metastasis and side population phenotype acquisition of PDAC cells in vitro.

From: PCDH1 promotes progression of pancreatic ductal adenocarcinoma via activation of NF-κB signalling by interacting with KPNB1

Fig. 2: PCDH1 promotes the growth, metastasis and side population phenotype acquisition of PDAC cells in vitro.

A Knockdown efficiency and enforced expression of PCDH1 in PDAC cell lines was evaluated by Western blotting. B An MTT assay was performed to detect the effect of PCDH1 on the proliferation of PDAC cell lines. C Representative images showing the effects of PCDH1 expression knockdown (left) or overexpression (right) on colony formation. The number of colonies containing more than 50 cells was counted. D The effects of PCDH1 knockdown (left) or overexpression (right) on migration and invasion were evaluated by Transwell assays. E Representative images showing the effects of PCDH1 knockdown on SP phenotype acquisition. The results are presented as the mean ± SD of three independent experiments. The data were assessed by Student’s 2-tailed t test. *P < 0.05, **P < 0.01.

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