Fig. 1: SALIS is higher-expressed in HCC tumors and cells.

a The top 10 upregulated and downregulated LncRNAs from GSE101728 were clustered and shown in heat map. SALIS was highlighted using a red box. b The expression of SALIS in HCC (374 cases) compared with normal tissues (50 cases). The original expression data is from The Cancer Genome Atlas Program (TCGA) database. c Detection of the expression levels of SALIS in 4 paired clinicl samples of HCC and adjacent non-tumor tissues by qPCR. d Comparison of SALIS expression between normal hepatic tissue and HCC cell lines (HepG2 and HCCLM3) by qPCR. The qPCR data represent the average of three independent experiments ±s.d. e in situ hybridization (ISH) detection of SALIS in HCC tissue array and adjacent normal specimens. Representative images with various levels of staining (negative or weak from normal tissues, strong from tumor tissues) are shown. Scale bar: 50 μM. f Association of SALIS expression scores with AJCC tumor stages (Phase I, II, III, and IV). Data are plotted as the means of 95% confidence interval ± s.d. *P < 0.05, **P < 0.01, ***P < 0.001.