Fig. 1: Identification of exosomes and the effect of GSCs-derived exosomes on the aggressiveness of glioma cells. | Cell Death & Disease

Fig. 1: Identification of exosomes and the effect of GSCs-derived exosomes on the aggressiveness of glioma cells.

From: Exosomal miR-155-5p derived from glioma stem-like cells promotes mesenchymal transition via targeting ACOT12

Fig. 1

A Respective transmission electron microscopy analysis of exosomes secreted by GSCs and HEB cells. Scale bar, 200 nm; B The particle size of isolated exosomes was measured by NTA; C Representative confocal fluorescence microscopy image showing the internalization of PKH26 (red)-labeled exosomes by glioma cells. Scale bar, 100 μm; D Exosome surface markers CD63 and CD9 and the negative control marker GM130 were detected by western blotting; E Wound healing assays in U87 and U251 cells after co-culture with GSCs; F Transwell assays in U87 and U251 cells after co-culture with GSCs. G Wound healing assays in U87 and U251 cells incubated with GSCs-derived exosomes or HEB-derived exosomes; H Transwell assays in U87 and U251 cells incubated with GSCs-derived exosomes or HEB-derived exosomes. Representative images are shown. Data were from three independent experiments and expressed as the mean ± SEM; **P < 0.01; ***P < 0.001; ****P < 0.0001 (Student’s t-test).

Back to article page