Fig. 4: Overexpression of EBV-miR-BART22 promotes the migration, invasion, and EMT of NPC cells. | Cell Death & Disease

Fig. 4: Overexpression of EBV-miR-BART22 promotes the migration, invasion, and EMT of NPC cells.

From: Epstein–Barr virus-encoded microRNA BART22 serves as novel biomarkers and drives malignant transformation of nasopharyngeal carcinoma

Fig. 4

A A representative bright-field image of the livers was shown. Liver weights and metastatic modules number in nude mice that received transplants of indicated cells. Bars correspond to mean ± standard deviation (SD), **P < 0.01. ***P < 0.001. (B). Representative H&E staining of indicated spleen orthotopic tumors is shown. C CNE-1, CNE-2, and SUNE-1 cells and appropriate controls were used in a wound-healing assay. D Quantification of indicated migrating cells in 5 random fields analyzed by Transwell assays, respectively. E Quantification of indicated invasion cells in 5 random fields analyzed by invasion assays, respectively. F Representative micrographs of indicated cells grown on matrigel for 10 days in 3D spheroid invasion assay. G Cells morphology was evaluated by phase-contrast microscopy. H Expression of epithelial cell marker and mesenchymal cell marker in indicated cells were examined by WB analysis. β-actin was used as a loading control.

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