Fig. 1: FOXJ2 localized to spermatocytes, round spermatids, and Sertoli cells in the seminiferous tubules and was highly expressed in spermatocytes. | Cell Death & Disease

Fig. 1: FOXJ2 localized to spermatocytes, round spermatids, and Sertoli cells in the seminiferous tubules and was highly expressed in spermatocytes.

From: Germline FOXJ2 overexpression causes male infertility via aberrant autophagy activation by LAMP2A upregulation

Fig. 1: FOXJ2 localized to spermatocytes, round spermatids, and Sertoli cells in the seminiferous tubules and was highly expressed in spermatocytes.The alternative text for this image may have been generated using AI.

A Expression and localization of FOXJ2 in mouse testes using immunohistochemical staining (IHC). The roman numbers present the stages of the seminiferous epithelia. S Sertoli cell, Rs round spermatid, L leptotene spermatocyte, M metaphase of spermatocyte. Scale bar: 10 μm. B Immunofluorescent staining of SCP3 (showing spermatocytes) and FOXJ2, PNA (showing acrosomes) and FOXJ2, WT1(showing Sertoli cells) and FOXJ2 on testis sections of 8-week-old WT mice. Nuclei were counterstained with DAPI. Long white arrows indicate spermatocytes (Spc). White solid arrowheads indicate round spermatids (Rs). White hollow arrowheads indicate elongating spermatids (Es). Short white arrows indicate meiotic metaphase spermatocytes (M). White thick arrows indicate Sertoli cells (S). Scale bar: 50 μm. C Expression and localization of FOXJ2 in isolated spermatocytes (Spc) and round spermatids (Rs) using immunofluorescent staining (IF). D Western blotting analysis of FOXJ2 protein levels in isolated spermatocytes (Spc) and round spermatids (Rs) with the corresponding average gray levels. H3 was used as a loading control. Data are presented as the mean ± SD (n = 3). *P < 0.05.

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