Fig. 7: LAMP2 co-localized with FOXJ2 in spermatocytes and was a target of FOXJ2. | Cell Death & Disease

Fig. 7: LAMP2 co-localized with FOXJ2 in spermatocytes and was a target of FOXJ2.

From: Germline FOXJ2 overexpression causes male infertility via aberrant autophagy activation by LAMP2A upregulation

Fig. 7: LAMP2 co-localized with FOXJ2 in spermatocytes and was a target of FOXJ2.The alternative text for this image may have been generated using AI.

A Expression and localization of LAMP2 in testis using immunohistochemical staining (IHC). Scale bar: 50 μm. B Localization of LAMP2 and SCP3 (showing spermatocytes) on testis sections using immunofluorescent staining. Scale bar: 50 μm. White arrows indicate spermatocytes (Spc). C Localization of LAMP2 and FOXJ2 in isolated spermatocytes using immunofluorescent staining (IF). Scale bar: 50 μm. D The binding sites of FOXJ2 to Lamp2 promoter region in the testicular cells of the 10-day-old Stra8-cre; Foxj2 tg/tg mice analyzed by ChIP-PCR using anti-HA-tag (ChIP level). Data are presented as the mean ± SD (n = 3). *P < 0.05. E Verification of FOXJ2 binding to the Lamp2 promoter using a Dual-luciferase reporter assay. Data are presented as the mean ± SD (n = 3). ***P < 0.001; ****P < 0.0001.

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