Fig. 1: The level of Sestrin2 is decreased in glomeruli from mice with DKD and renal biopsies from individuals with DKD.

A Representative immunohistochemical staining images of Sestrin2 in renal biopsies from normal subjects and diabetic kidney disease (DKD) patients. Scale bar: black 100 μm, red 50 μm. B Representative immunofluorescence images of Sestrin2 (green) and synaptopodin (red) in glomeruli from wild-type (WT) mice and STZ-induced diabetic (WT + DM) mice of 8 weeks, 12 weeks or 16 weeks. C Quantification of Sestrin2 in human renal biopsies (five glomeruli were analyzed, n = 5). D Quantification of Sestrin2 in glomeruli from all groups of mice (five glomeruli were analyzed, n = 5). E The levels of Sestrin2 were examined by ELISA from serum of WT mice and mice of WT + DM group (n = 7). F, G The protein level of Sestrin2 was detected by western blot in mouse podocytes exposed to high glucose (HG) at time courses of 0, 3, 6, 12 and 24 h (**P < 0.01 vs HG 0 h, n = 3). Data are expressed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.