Table 1 The sequences of PCR primer pairs used in this study.
Gene | Forward primers (5′–3′) | Reverse primers (5′–3′) |
|---|---|---|
α-SMA | CGCCCTCGCCACCAGATCTG | TAGCCTTCATAGATGGGGAC |
E-cadherin | GCCGGAGCCCTGCCACCCTG | CTTTCTGTAGGTGGAGTCCC |
nephrin | GTTCCAAGCCAAAGGATGCC | GCATGTAATGCCAGGGCT |
desmin | CCATTGCCCTGGGATGAACT | TACCCGATGCCCAGGTGATA |
synaptopodin | GCCCTCCTTCTGCTTCAAGT | TCTTCCTCACTAAGCCCCGA |
Bax | GCTTGCTTCAGGGTTTCATCCA | TGTCCACGGCGGCAATCATC |
Bcl-2 | GGCGGAGAACAGGGTACGATAAC | CGGGATGCGGCTGGATGGGG |
TSP-1 | ATGCCTGCGATGATGACGATGAC | ATACTGGGCTGGGTTGTAATGGAATG |
CD36 | GTCTATCTACGCTGTGTTCGGATCTG | TGTCTGGATTCTGGAGGGGTGATG |
TGF-β1 | ACCGCAACAACGCCATCTATGAG | GGCACTGCTTCCCGAATGTCTG |
Smad3 | AGACGCCAGTTCTACCTCCAGTG | GCCAGCAGGGAAGTTAGTGTTCTC |
Nox4 | CGAGCCAAAGGGGCCCTGAAG | AACAGCGTGCGTCTAACGGCA |
18s | ACACGGACAGGATTGACAGA | GGACATCTAAGGGCATCACAG |
