Fig. 5: MLN 2238 activates JNK which is required for CREB activation in 293 T cells. | Cell Death & Disease

Fig. 5: MLN 2238 activates JNK which is required for CREB activation in 293 T cells.

From: The CRTC-CREB axis functions as a transcriptional sensor to protect against proteotoxic stress in Drosophila

Fig. 5

a CREB phosphorylation status (Ser131) in 293 T cells was detected by Western blot. Total protein lysate from control and cells treated with MLN2238 (5 nM) and/or SP600125 (40 uM) for 24 h by immunoblotting using pCREB, CREB and α-tubulin antibodies. Representative blots from three independent experiments are shown. b Relative expression of CREB and pCREB in immuno-blots shown in panel a were quantified using α-tubulin as internal controls. t-Test was performed for statistical analysis. **p < 0.01, n.s.: no significance. c Representative images of immune-staining against phospho-JNK(pJNK at Thr183 and Tyr185) and phospho-CREB(anti-CREB Ser133) after treat with MLN2238 (5 nM) and/or SP600125 (40 uM) for 24 h in 293 T cells. Separate channels shown on the right. Scale bar: 10um. Arrowhead points to cells accumulated with pCREB and pJNK after MLN2238 treatment.

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