Fig. 2: TMCO1 deficiency increases the migration of glial cells from the GW to the IG.
From: Ca2+ homeostasis maintained by TMCO1 underlies corpus callosum development via ERK signaling
A Upper: Immunofluorescence for the axonal marker LICAM (green) and the glial marker GFAP (red) in coronal sections of Tmco1+/+ and Tmco1−/− embryonic brains at E18.5. High magnification of the midline area (white box) is shown in lower panels. Scale bar, 500 μm. B SOX9 immunofluorescence (green) at the telencephalic midline in Tmco1+/+ and Tmco1−/− embryonic brains in rostral, middle and caudal coronal sections through the developing CC region. Scale bar, 100 μm. GW glia wedge, IG indusium griseum. C–E The counts of SOX9-positive cells in the GW (C), IG (D) and GW + IG (E). Tmco1+/+, n = 3; Tmco1−/−, n = 4. Two-tailed unpaired Student’s t test. F The timeline of the experiment for SOX9 immunostaining after BrdU injection. G BrdU (green) and SOX9 (red) co-immunofluorescence at the telencephalic midline of Tmco1+/+ and Tmco1−/− embryonic brains at E16.5 after a single BrdU administration at E14.5. Scale bar, 100 μm. IG indusium griseum. (H) Quantification of the number of BrdU+SOX9+ cells at the IG in Tmco1+/+ and Tmco1−/− midline. Tmco1+/+, n = 3; Tmco1−/−, n = 4. Two-tailed unpaired Student’s t test. (I) In situ hybridization for the expression of Slit2 in coronal sections from Tmco1+/+ and Tmco1−/− E16.5 embryos. Scale bar, 500 μm. See also Figs. S2 and S3.
