Fig. 2: KNTC1 knockdown inhibited NSCLC development in vitro.
From: shRNA‑mediated knockdown of KNTC1 inhibits non-small-cell lung cancer through regulating PSMB8
A The cell proliferation rate was evaluated in NSCLC cell lines after transfection by MTT assay. B The effects of KNTC1 knockdown on cell cycle were determined by flow cytometry. C The effects of KNTC1 knockdown on cell apoptosis were examined by flow cytometry. D Human apoptosis antibody array was utilized to illustrate the regulation of the expression of apoptosis-related proteins by KNTC1 knockdown in A549 cells transfected with or without KNTC1. Protein expression was presented in grayscale and visualized by R studio. E The migration rate of cells was detected in NSCLC cell lines after transfection by transwell assay. Magnification times: ×200. F The migration rate of cells was detected in NSCLC cell lines after transfection by wound-healing assay. G The interaction between KNTC1 and AKT pathway was verified via the IPA analysis. H The expression of some cancer-associated factors was detected by western blot. Results were presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.
