Fig. 3: Exploration the underlying mechanism of KNTC1 regulating NSCLC.
From: shRNA‑mediated knockdown of KNTC1 inhibits non-small-cell lung cancer through regulating PSMB8
A The heatmap of DEGs identified by RNA-sequencing of NCI-H1299 cells treated with shCtrl (n = 3) or shKNTC1 (n = 3). B The enrichment of the DEGs in canonical signaling pathways was analyzed by the IPA analysis. C, D The expression of several most significantly differentially expressed genes identified by qPCR (C) and western blot (D) in NCI-H1299 with shKNTC1. E MTT assay was performed to detect the levels of cell proliferation of NCI-H1299 cells after transfecting S100A10, BAG2, RRP9, DPP3, ZNF655 and PSMB8 corresponding lentiviruses. F The potential linkage of KNTC1, PSMB8 and Ephrin Receptor Signaling, Huntington’s Disease Signaling and Actin Cytoskeleton Signaling was illustrated via the IPA analysis. G The expression levels of PSMB8 in NSCLC tumor tissues and para-carcinoma tissues were determined by immunohistochemical staining. **P < 0.01.
