Fig. 2: CircKPNB1 overexpression promotes the malignant phenotype of GSCs in vitro. | Cell Death & Disease

Fig. 2: CircKPNB1 overexpression promotes the malignant phenotype of GSCs in vitro.

From: CircKPNB1 mediates a positive feedback loop and promotes the malignant phenotypes of GSCs via TNF-α/NF-κB signaling

Fig. 2

a The circKNPB1 expression in six patient-derived GSCs. b, c MTS assays showing the cell viabilities changes in circKNPB1 overexpressed GSC28 and GSC32. The absorbance value of two groups was compared by two-tailed Student’s t-test at different hours, respectively. d EDU assays showing the proliferation changes in circKNPB1 overexpressed GSC28 and GSC32. Scale bar = 100 μm. e Transwell assays showing the change of invasion cell numbers after circKNPB1 overexpression in GSC28 and GSC32. Scale bar = 50 μm. f Neuroshperes formation assays showing the neurospheres formation abilities after circKNPB1 overexpression in GSC28 and GSC32. Scale bar = 50 μm. g, h Limiting dilution assays showing the self-renewing capacity of GSC4D circKNPB1 overexpressed GSC28 and GSC32. All data are expressed as the mean ± SD (three independent experiments). *p < 0.05; **p < 0.01; ***p < 0.001.

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