Fig. 6: DGCR8 can bind to and maintain the stability of circKPNB1. | Cell Death & Disease

Fig. 6: DGCR8 can bind to and maintain the stability of circKPNB1.

From: CircKPNB1 mediates a positive feedback loop and promotes the malignant phenotypes of GSCs via TNF-α/NF-κB signaling

Fig. 6

a The RBPs of circKPNB1 were predicted by circIntercome and CSCD. b RPISeq showing the binding interaction possibilities between circKPNB1 and four RBPs (TIAL1, IGF2BP3, DGCR8 and FUS). c–f qPCR showing circKPNB1 expression after overexpression (c, d) or knockdown (e, f) of the four RBPs (TIAL1, IGF2BP3, DGCR8 and FUS). g, h RIP assays showing anti-DGCR8 treatment enriched with circKPNB1 after circKPNB1 overexpression (g) or knockdown (h). i, j RNA pull-down assays showing the biotinylated circKPNB1 probes pull-down DGCR8 protein. k, l RNA stability assays showing the half-life of circKPNB1 in DGCR8 overexpressed GSCs after actinomycin D treatment. All data are expressed as the mean ± SD (three independent experiments). *p < 0.05; **p < 0.01; ***p < 0.001.

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