Fig. 4: FOXA1 interacted with DNMT1 and mediated the loss of imprinting of IGF2 in LUAD cells. | Cell Death & Disease

Fig. 4: FOXA1 interacted with DNMT1 and mediated the loss of imprinting of IGF2 in LUAD cells.

From: FOXA1 prevents nutrients deprivation induced autophagic cell death through inducing loss of imprinting of IGF2 in lung adenocarcinoma

Fig. 4: FOXA1 interacted with DNMT1 and mediated the loss of imprinting of IGF2 in LUAD cells.

A Heat map of differentially expressed genes induced by gain or loss of function of FOXA1 in LUAD cells. B RT-PCR assays. C Western blot assays. D ChIP-seq data visualized using IGV software. E FOXA1 binding to IGF2-ICR measured using ChIP-qPCR assays. Mean ± SD, n = 3. F Methylation status of IGF2-ICR LUAD cells as evaluated by MSP assays. G Co-immunoprecipitation assays. H Co-localization of FOXA1 with DNMT1 visualized using immunofluorescence assays. Scale bar: 10 μm. I, J DNMT1 or CTCF binding to the CpG sites of IGF2-ICR determined by ChIP-qPCR. Mean ± SD, n = 3. K, L Effects of 5-Aza on IGF2 mRNA and protein levels measured by qPCR and western blot assays. Mean ± SD, n = 3. M, N Effects of silencing DNMT1 on IGF2 mRNA and protein levels measured by qPCR and western blot assays. Mean ± SD, n = 3. *P < 0.05; **P < 0.01; ***P < 0.001, NS not significant.

Back to article page