Fig. 6: Repression of GBA1 protein by IGF2 signaling contributed to the survival advantage of LUAD cells conferred by FOXA1 under metabolic stress conditions. | Cell Death & Disease

Fig. 6: Repression of GBA1 protein by IGF2 signaling contributed to the survival advantage of LUAD cells conferred by FOXA1 under metabolic stress conditions.

From: FOXA1 prevents nutrients deprivation induced autophagic cell death through inducing loss of imprinting of IGF2 in lung adenocarcinoma

Fig. 6: Repression of GBA1 protein by IGF2 signaling contributed to the survival advantage of LUAD cells conferred by FOXA1 under metabolic stress conditions.

A The mRNA levels of GBA1 were evaluated by RT-PCR. B The protein levels of GBA1 in LUAD cells were measured by western blotting. C, D The mRNA and protein levels of GBA1 in siRNA-transfected LUAD cells were measured by RT-PCR and western blot assays. Mean ± SD, n = 3. E Effect of silencing GBA1 on cell growth under nutrient-rich conditions was evaluated by using CCK-8 assays. Mean ± SD, n = 5. F Effects of silencing GBA1 on survival in LUAD cells under nutrient-rich or starvation conditions were measured using colony formation assays. Mean ± SD, n = 3. G, H The mRNA and protein levels of GBA1 in FOXA1-expressing or FOXA1-deficient cells were measured by RT-PCR and western blot assays. I GBA1 protein levels in FOXA1-expressing A549 cells treated with or without MG132 as determined by western blot assays. J Colony formation assays. Mean ± SD, n = 3. K, L Effects of IGF2 on GBA1 mRNA and protein levels were determined by RT-PCR or western blot assays. Mean ± SD, n = 3. M, N Effects of IGF2 on GBA1 mRNA and protein levels in FOXA1-depleted PC-9 cells were determined by RT-PCR or western blot assays. O, P Effects of linsitinib on endogenous GBA1 levels in PC-9 cells were determined by RT-PCR and western blot assays. Q, R Effects of linsitinib and rapamycin on GBA1 levels in FOXA1-expressing A549 cells were determined by RT-PCR or western blot assays. Mean ± SD, n = 3. S Ubiquitination of GBA1 was determined by immunoprecipitation and western blot assay. *P < 0.05; **P < 0.01; ***P < 0.001.

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