Fig. 2: Arecoline can upregulate the expression levels of PA28γ and phosphorylated MEK1 in epithelial cells, which induce EMT. | Cell Death & Disease

Fig. 2: Arecoline can upregulate the expression levels of PA28γ and phosphorylated MEK1 in epithelial cells, which induce EMT.

From: Identification of a BRAF/PA28γ/MEK1 signaling axis and its role in epithelial-mesenchymal transition in oral submucous fibrosis

Fig. 2: Arecoline can upregulate the expression levels of PA28γ and phosphorylated MEK1 in epithelial cells, which induce EMT.

Western blot assays showed that arecoline upregulated the expression levels of PA28γ, p-MEK1, and p-ERK in an adosage-dependent (A) and time-dependent manner (B). C After 30 days of treatment of epithelial cells with low concentrations of arecoline (10 μg/mL), the morphology of epithelial cells transformed to a mesenchymal phenotype. Scale bar: 25 μm. Wound healing (D, E) and transwell assays F, G showed that the healing ability and migration ability of epithelial cells treated with arecoline were upregulated. Scale bar: 100 μm. Data represent the means ± s.d. of three independent experiments. Statistical analysis was performed using Student’s t test (*p < 0.05, ***p < 0.001). H Representative results for immunostaining of PA28γ, Vimentin, and E-cadherin in the normal control group and the arecoline-treated group. Scale bar: 50 μm. I Western blot assays showed the expression levels of Vimentin and E-cadherin in arecoline-treated epithelial cells.

Back to article page