Fig. 3: PA28γ can bind to MEK1 and activate the MEK1/ERK signaling pathway. | Cell Death & Disease

Fig. 3: PA28γ can bind to MEK1 and activate the MEK1/ERK signaling pathway.

From: Identification of a BRAF/PA28γ/MEK1 signaling axis and its role in epithelial-mesenchymal transition in oral submucous fibrosis

Fig. 3: PA28γ can bind to MEK1 and activate the MEK1/ERK signaling pathway.The alternative text for this image may have been generated using AI.

A, B HA-tagged MEK1 and Flag-tagged PA28γ plasmids were transfected into 293T cells, and immunoprecipitation experiments showed that PA28γ and MEK1 have a direct interaction relationship. Immunoprecipitation of MEK1 was performed with the HA-MEK1 antibody. C, D Coimmunoprecipitation assays showed that endogenous PA28γ and endogenous MEK1 could bind to each other. Immunoprecipitation of PA28γ was performed with the anti-PA28γ or anti-Flag antibody. E, F Representative results of the coimmunostaining of Flag-PA28γ (red) and endogenous MEK1 (green) in epithelial cells. The nuclei were stained blue with DAPI. Scale bar: 50 μm. G After the Flag-tagged PA28γ plasmid was transfected into 293T cells in a dose-dependent manner, western blot assays showed that the expression levels of p-MEK1 and p-ERK were upregulated. H Western blot assays showed that the epithelial cells were treated with siRNA-PA28γ, siRNA-MEK1, or siNC.

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