Fig. 6: BRAF inhibited the ubiquitination level of PA28γ.

293T cells were constantly transfected with vector (2 μg), Flag-PA28γ (2 μg), or Myc-BRAF plasmid (2 μg) 24 h later, and then serum-starved 293T cells were pretreated with A CHX (100 μg/ml) or B MG132 (10 μM) for the indicated periods of time. Data represent the means ± s.d. of three independent experiments. Quantification of Flag-PA28γ levels relative to GAPDH is shown. Statistical analysis was performed using Student’s t test (**p < 0.01). C The ubiquitylation assay showed the ubiquitination degradation process of the PA28γ protein. D, E BRAF inhibited the PA28γ ubiquitination level at both the K48 and K63 sites.