Fig. 5: METTL3 mediates m6A modification of PTEN and regulates its expression through reading protein YTHDF2. | Cell Death & Disease

Fig. 5: METTL3 mediates m6A modification of PTEN and regulates its expression through reading protein YTHDF2.

From: METTL3 promotes prostatic hyperplasia by regulating PTEN expression in an m6A-YTHDF2-dependent manner

Fig. 5

A METTL3 overexpression or knockdown was achieved in BPH-1 cells by transfecting OE-METTL3 or sh-METTL3 and confirmed using qRT-PCR. BPH-1 cells were transduced with OE-METTL3 or sh-METTL3 and examined for PTEN mRNA expression using qRT-PCR (B); PTEN protein levels using Immunoblotting (C). The m6A modification status of PTEN using Me-RIP assay (D); PTEN mRNA synthesis using actinomycin D assay (E). **P < 0.01, compared with the vector group; ##P < 0.01, compared with the sh-NC group. F The binding between YTHDF2 and PTEN was validated in BPH-1 cells using RIP assay with anti-IgG and anti-YTHDF2. ***P < 0.001, compared with the anti-IgG group. G The binding between YTHDF2 and PTEN was validated in sh-NC or sh-METTL3 transduced BPH-1 cells using RIP assay with anti-IgG and anti-YTHDF2. *P < 0.05, compared with the sh-NC group. (H, I) YTHDF2 overexpression or knockdown was achieved in BPH-1 cells by transfecting OE-YTHDF2 or sh-YTHDF2 and confirmed using qRT-PCR and Immunoblotting, respectively. (J, K) BPH-1 cells were transduced with OE-YTHDF2 or sh-YTHDF2 and examined for PTEN mRNA expression and protein levels using qRT-PCR and Immunoblotting, respectively. **P < 0.01, compared with the vector group; ##P < 0.01, compared with the sh-NC group.

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