Fig. 6: METTL3 regulates PTEN and affects cell proliferation and EMT in prostatic hyperplasia model rats. | Cell Death & Disease

Fig. 6: METTL3 regulates PTEN and affects cell proliferation and EMT in prostatic hyperplasia model rats.

From: METTL3 promotes prostatic hyperplasia by regulating PTEN expression in an m6A-YTHDF2-dependent manner

Fig. 6

BPH model was established in rats as described above; rats were injected with Lv-sh-METTL3 and/or Lv-sh-PTEN and examined for A–C the mRNA and protein expressions of METTL3 and PTEN in rats’ prostate using qRT-PCR and Immunoblotting; D–E prostatic epithelial thickness and prostate weight at the end of the modeling and treatment; F a representative prostate with the urinary bladder from each group (upper) and the histopathological characteristics of rats’ prostate from each group using H&E staining (down); Scale bar = 1 cm or 100 μm; G–K the contents of Ki67, PCNA, E-cadherin, N-cadherin, and vimentin in rats’ prostate tissues using Immunohistochemical (IHC) staining; L cell apoptosis in rats’ prostate using TUNEL assay; scale bar = 50 μm. **P < 0.01, compared with the Lv-sh-NC group. ##P < 0.01, compared with the Lv-sh-METTL3 group.

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