Fig. 4: Analysis of the correlation between N1-ICD and GSK3β inactivation levels in CLL cells. | Cell Death & Disease

Fig. 4: Analysis of the correlation between N1-ICD and GSK3β inactivation levels in CLL cells.

From: GSK3β is a critical, druggable component of the network regulating the active NOTCH1 protein and cell viability in CLL

Fig. 4

Left, the expression of N1-ICD, pS9-GSK3β used as a marker of GSK3β inactivation status, total GSK3β, and GAPDH used as a loading control, was examined by Western blot analysis (n = 30). Four representative samples are shown. Right, quantification of N1-ICD bands, normalized to GAPDH levels, and of pS9-GSK3β bands normalized to total GSK3β levels, was performed by densitometric analysis. Correlation between N1-ICD and pS9-GSK3β expression values was assessed by using the Spearman’s correlation coefficient (r). P < 0.0001.

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