Fig. 3: Upregulated CD36 induces podocyte injury via activating NLRP3 inflammasome. | Cell Death & Disease

Fig. 3: Upregulated CD36 induces podocyte injury via activating NLRP3 inflammasome.

From: CD36 aggravates podocyte injury by activating NLRP3 inflammasome and inhibiting autophagy in lupus nephritis

Fig. 3

A Western blot analysis of CD36, Nephrin, and Podocin in human podocytes at different IgG-LN and IgG-Control concentrations (exposure to IgG-LN for 72 h). B Western blots analysis of CD36, Nephrin, and Podocin in human podocytes after exposure to 0.5 μg/ml IgG-LN and IgG-Control at various time points. C Western blot analysis of CD36, Nephrin, Podocin, NLRP3, Caspase1, Cleaved-Caspase1, and IL-1β in human podocytes with treatment of IgG-LN (500 μg/ml; 72 h) (Untreated vs. IgG-LN). D Representatives of Annexin V-PI double-staining in different groups and quantitative of apoptosis was shown in histogram with mean ± SD from 3 experiments. (Untreated vs. IgG-LN). E The effect of CD36 knockout on LN-related podocyte injury was explored by CRISPR/Cas9 technology and subsequent western blot analysis (Untreated vs. IgG-LN; IgG-LN vs. CD36 KO + IgG-LN). F Representatives of Annexin V - PI double-staining in different groups and quantitative of apoptosis was shown in histogram with mean ± SD from 3 experiments (Untreated vs. IgG-LN; IgG-LN vs.CD36 KO + IgG-LN). G Western blot analysis of CD36, Nephrin, Podocin, NLRP3, Caspase1, Cleaved-Caspase1, and IL-1β in human podocytes under different treatments (Untreated vs. IgG-LN; IgG-LN vs. CD36 KO + IgG-LN; CD36 KO + IgG-LN vs. CD36 KO + CD36 OE + IgG-LN). Cle-Caspase1: Cleaved-Caspase1. Data are expressed as the mean ± SD. *p < 0.05. **p < 0.01. ***p < 0.001.

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