Fig. 4: Ivermectin repressed E2F targets.
From: Integrated analysis reveals FOXA1 and Ku70/Ku80 as targets of ivermectin in prostate cancer
A Normalized-enrichment scores (NES) of GSEA hallmark gene sets for all four comparation in C4-2 and 22RV1 cells. Significant gene sets comparing ivermectin versus vehicle (P value < 0.05) are labeled. B Venn diagram indicating the number of DEGs between C4-2 and 22RV1 cells. C, D The GSEA of C4-2 and 22RV1 concordant altered genes highlighted that hallmark E2F targets (C) and TRANSFAC E2F1 targets (D) were repressed by ivermectin. E, F The protein (E) and mRNA (F) expression of E2F1 decreased in C4-2 and 22RV1 cells treated with ivermectin. G Western blots showing thermostable E2F1 following indicated heat shocks in the presence (+) or absence (−) of 50 μM ivermectin in C4-2 cells.
