Fig. 4: Hsa_circ_0007367 functions as a sponge for miR-6820-3p in PDAC. | Cell Death & Disease

Fig. 4: Hsa_circ_0007367 functions as a sponge for miR-6820-3p in PDAC.

From: Circular RNA hsa_circ_0007367 promotes the progression of pancreatic ductal adenocarcinoma by sponging miR-6820-3p and upregulating YAP1 expression

Fig. 4

A Schematic drawing showing potential target miRNAs of hsa_circ_0007367 predicted by starbase, circbank, and circular RNA interactome. B The relative expression of seven potential target miRNAs in PANC-1 and AsPC-1 cells lysates were detected by qRT-PCR. MiR-6820-3p was stably pulled down by hsa_circ_0007367. C Schematic illustration of the wild-type (WT) and mutant (mut) hsa_circ_0007367 luciferase plasmid. D The luciferase activities of the hsa_circ_0007367 luciferase reporter vector (WT or mut) in HEK293T cells transfected with miR-6820-3p mimics or mimic NC. E, F qRT-PCR detected the relative expression of miR-6820-3p in PDAC tissues (E) and in HPDE and PANC-1 cell lines (F). G The relative expression of hsa_circ_0007367 in PANC-1 cells was detected by qRT-PCR after hsa_circ_0007367 knocking down. H Correlation analysis between hsa_circ_0007367 and miR-6820-3p expression in PDAC tissues. All data are shown as the mean ± SD of at least three independent experiments. *p < 0.05, **p < 0.01.

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