Fig. 3: SEAS1 overexpression suppresses proliferation, invasion, and facilitates apoptosis of TNBC cells.
From: LncRNA SEMA3B-AS1 inhibits breast cancer progression by targeting miR-3940/KLLN axis
A–C CCK8, colony formation, and EdU assay results show the proliferation rates of control and SEAS1 overexpressing MDA-MB-231 and BT-549 cells. D Flow cytometry results show the apoptotic rates in the control and SEAS1 overexpressing MDA-MB-231 and BT-549 cells. E Transwell assays show the migration and invasion rates of control vector-transfected and SEAS1-overexpression vector-transfected MDA-MB-231 and BT-549 cells. The data represent average of three independent experiments. F Western blot analysis shows the relative expression levels of EMT-related proteins, namely, N-cadherin, Vimentin, Snail, and E-cadherin in the control, SEAS1-silenced, and SEAS1-overexpressing MDA-MB-231 or BT-549 cells. *P < 0.05, **P < 0.01, and ***P < 0.001. Representative data from at least 3 experiments with comparable results are shown.
