Fig. 7: LINC00239 expression has a positive correlation with Nrf2 and GPX4 expression in CRC tissues.

A Representative images of IHC or ISH staining of LINC00239, Nrf2, and GPX4 expression in human CRC samples in Cohort I (n = 174). The scale bars represent 250 μm (low magnification) and 50 μm (high magnification). B Correlation analysis of LINC00239 and Nrf2 expression in the CRC tissues in cohort I (n = 174). C Correlation analysis of LINC00239 and GPX4 expression in the CRC tissues in cohort I (n = 174). D, E Kaplan–Meier’s curves generated with the data from the CRC patients with negative versus positive LINC00239, Nrf2, or GPX4 expression. The correlation between LINC00239 and Nrf2 (D) or GPX4 (E) expression and overall survival or recurrence in patients with CRC in cohort I (n = 174). F Representative images of IHC or ISH staining of LINC00239, Nrf2, and GPX4 expression in human CRC samples in cohort II (n = 180). The scale bars represent 250 μm (low magnification) and 50 μm (high magnification). G Correlation analysis of LINC00239 and Nrf2 expression in the CRC tissues in cohort II (n = 180). H Correlation analysis of LINC00239 and GPX4 expression in the CRC tissues in cohort II (n = 180). I, J Kaplan–Meier’s curves generated with the data from the CRC patients with negative versus positive LINC00239, Nrf2 or GPX4 expression. The correlation between LINC00239 and Nrf2 (I) or GPX4 (J) expression and overall survival or recurrence in patients with CRC in cohort II (n = 180). K, L Real-time PCR of LINC00239 and GPX4 expression in adjacent nontumor samples and CRC samples (n = 22). M LINC00239 expression positively correlated with the expression levels of GPX4 in CRC samples (n = 22). N Proposed model of the relationship between LINC00239 and Nrf2 in CRC cells. Data shown represent mean ± SD from three independent experiments. ns P > 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, Student’s t test.