Fig. 5: RNAi of lola induces ovarian apoptosis. | Cell Death & Disease

Fig. 5: RNAi of lola induces ovarian apoptosis.

From: A dual role of lola in Drosophila ovary development: regulating stem cell niche establishment and repressing apoptosis

Fig. 5

A Ovaries from the c587 > w1118 control line (a) and c587 > lola12574 RNAi line reared at 25 °C from eggs to adults (b, c) were stained with TUNEL assay for apoptotic cells (a’–c’, green), and nuclei were stained with DAPI (a”–c”, white). In a control ovary, cells were TUNEL-negative (a–a”), while in c587 > lola12574 RNAi ovaries, TUNEL-positive was observed (b–b”, c–c”). B The c587 > w1118 and c587 > lola12574 RNAi lines were reared at 18 °C from eggs to pupae and then at 25 °C from pupae to adults. Ovaries from 3-4 days c587 > w1118 and c587 > lola12574 RNAi adults were dissected and stained with DAPI. DNA fragments were detected in the ovaries of c587 > lola12574 RNAi adults (b and c, circled in yellow), but not in the ovaries of c587 > w1118 control adults (a). Scale bar: 100 µm.

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