Fig. 3: The promoting effect of GASP1 on the invasiveness of breast cancer cells.

a GASP1 knockout suppressed the migration and invasion potential of HCC1937 and MCF7 cells. The representative pictures of migrated/invaded cells were shown in the left panels, and statistical data of cell numbers was shown in the right panels. b GASP1 knockout down-regulated the expression of several metastasis-related genes in HCC1937 and MCF7 cells compared with the control, including N-cadherin, MMP9, MMP2, Slug, and snail. c GASP1 overexpression enhanced the migration and invasion potential of MDA-MB-231 and DU4475 cells. The representative pictures were shown in the left panels and migrated/invaded cell quantification was shown in the right panels. d Ectopic expression of GASP1 in MDA-MB-231 and DU4475 cells up-regulated the expression of N-cadherin, MMP9, MMP2, Slug, and snail. Data were presented as mean ± SD. ***P < 0.0001.