Fig. 6: A reduction in ceramide levels and its interaction with PTEN activates AKT/ERK signalling to promote angiogenesis.

A, B p-AKT, AKT, p-ERK, ERK and PTEN expression levels in HAECs (A), ECs-shS1PR1 (B) and ECs transfected with CerS3 overexpression plasmids (B) were determined by WB. C An interaction between endogenous ceramide and PTEN was detected by PLA in ECs with S1PR1 knockdown. BF bright field.