Fig. 1: Syn1 is palmitoylated at Cys-223, Cys-360, and Cys-370. | Cell Death & Disease

Fig. 1: Syn1 is palmitoylated at Cys-223, Cys-360, and Cys-370.

From: Crosstalk of Synapsin1 palmitoylation and phosphorylation controls the dynamicity of synaptic vesicles in neurons

Fig. 1: Syn1 is palmitoylated at Cys-223, Cys-360, and Cys-370.

A, B Syn1 expressed in HEK-293T cells or lysate of mice hippocampi was analyzed for protein palmitoylation by Acyl-RAC assay. HA+, with NH2OH, HA-, without NH2OH. C, D HEK-293T cells expressing Syn1-his was incubated with 50 μM 2-BP for 8 h and evaluated for the level of palm-Syn1. ***p < 0.001; t-test (n = 4 biological replicates). E Protein sequences of Syn1 from various species were aligned for analyzing cysteine conservation. F Purified Syn1-flag was probed by mass-spectrometry, a mass-shift of 238 Da linked to cysteine is a hallmark for palmitoylation, suggesting that cysteine-360 is palmitoylated. G, H His-tagged Syn1-WT and its mutants were analyzed by Acyl-RAC for the level of palm-Syn1. **p < 0.01; ***p < 0.001; ****p < 0.0001; one-way ANOVA; n = 4 biological replicates, Data are mean ± s.e.m.

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