Fig. 5: SEMA4B-211aa interacts with p85 and regulates PI3K activity. | Cell Death & Disease

Fig. 5: SEMA4B-211aa interacts with p85 and regulates PI3K activity.

From: CircSEMA4B inhibits the progression of breast cancer by encoding a novel protein SEMA4B-211aa and regulating AKT phosphorylation

Fig. 5: SEMA4B-211aa interacts with p85 and regulates PI3K activity.

a Expression of key proteins in PI3K pathway were detected in BC cells transfected with Flag-circSEMA4B, Flag-circSEMA4B-MUT, Flag-circSEMA4B-NC, and Flag-circSEMA4B-211aa. b Mutual interaction of p85 and SEMA4B-211aa were determined by Co-IP. c p85/p110 complex was influenced by SEMA4B-211aa by Co-IP. d Colocalization of p85 and SEMA4B-211aa in BC cells. Red, SEMA4B-211aa; green, p85; blue, DAPI. e, f PI3K activity was determined by ELISA according to PIP2/PIP3 expression in BC cells transfected with Flag-circSEMA4B, Flag-circSEMA4B-MUT, Flag-circSEMA4B-NC, and Flag-circSEMA4B-211aa.

Back to article page