Fig. 2: Knockdown of FBXO22 inhibits cell growth and G1/S phase progression, and induces apoptosis.
From: Fbxo22 promotes cervical cancer progression via targeting p57Kip2 for ubiquitination and degradation
A, B The relative mRNA (A) and protein levels (B) of FBXO22 in one human immortalized cervical cell line (H8) and five various human CC cell lines by qRT-PCR and Western blotting method, respectively. The FBXO22 protein level was presented as the intensity of FBXO22 band normalized to that of the corresponding GAPDH band of each CC cell line (B, right panel). *P < 0.05, **P < 0.01, ***P < 0.001. Data are shown as mean ± SEM from triplicate measurements. C, D The mRNA level (C) and protein level (D) of FBXO22 were detected after stable expression of FBXO22 shRNAs (shR1 and shR2) or control shRNA (shCtr) in SiHa and C33A cells. GAPDH level was used as a loading control. **P < 0.01, ***P < 0.001. Data are shown as mean ± SEM from triplicate measurements. E, F FBXO22 knockdown inhibited cells viability (E) and colony formation (F) in SiHa and C33A cell lines. *P < 0.05, ***P < 0.001. Data are shown as mean ± SEM from triplicate measurements. G, H Knockdown of FBXO22 induced the apoptosis (G) in SiHa and C33A cells and inhibited the G1/S phase cell cycle progression (H) in SiHa cells. Each data in the plots represents the means ± SEM from triplicate measurements. *P < 0.05, **P < 0.01, ***P < 0.001.
