Fig. 3: HOXD11 promote cell proliferation and invasion in PSCC in vitro. | Cell Death & Disease

Fig. 3: HOXD11 promote cell proliferation and invasion in PSCC in vitro.

From: miR-138-5p-mediated HOXD11 promotes cell invasion and metastasis by activating the FN1/MMP2/MMP9 pathway and predicts poor prognosis in penile squamous cell carcinoma

Fig. 3: HOXD11 promote cell proliferation and invasion in PSCC in vitro.

After overexpressing or silencing HOXD11, PSCC cells were assessed for cell proliferation, clone formation, cell migration and invasion. Besides, rescue experiments were performed to determine to the oncogenic phenotype. A WB analysis for HOXD11 expression. B CCK-8 assays to determine the proliferative potential of HOXD11. C WB was performed to detect the rescue efficacy when HOXD11 was overexpressed in shHOXD11 cells. D Colony formation assay, E transwell invasion assays F and wound healing assays showed that the dysregulation of HOXD11 was involved in tumorigenesis in vitro. G, H Overexpression of HOXD11 restored the inhibition of cell migration and invasion in shHOXD11 cells. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Statistics are presented as the means ± SDs of three independent experiments. NS, not significant; NC, negative control; PSCC, penile squamous cell carcinoma.

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