Fig. 4: Knockdown of HOXD11 inhibited tumor growth and popliteal lymph node metastasis in vivo. | Cell Death & Disease

Fig. 4: Knockdown of HOXD11 inhibited tumor growth and popliteal lymph node metastasis in vivo.

From: miR-138-5p-mediated HOXD11 promotes cell invasion and metastasis by activating the FN1/MMP2/MMP9 pathway and predicts poor prognosis in penile squamous cell carcinoma

Fig. 4: Knockdown of HOXD11 inhibited tumor growth and popliteal lymph node metastasis in vivo.

Penl2 HOXD11-sh1 cells were injected subcutaneously to establish xenograft tumor model in BALB/c nude mice (n = 6) for three weeks. A Knockdown of HOXD11 inhibited tumor growth in vivo. B IHC was performed to detect the expression of HOXD11 in two groups. The mRNA expression of HOXD11 was knockdown in subcutaneous tumors of the shHOXD11 group. C The effect of HOXD11 on lymph nodes metastasis was explored by an inguinal and popliteal lymph node metastasis model (n = 6). Knockdown of HOXD11 inhibited the volume of popliteal lymph node, and HE staining demonstrated the reduction of lymph node metastasis. *p < 0.05. NC, negative control; HE, hematoxylin-eosin; IHC, immunohistochemistry.

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