Fig. 5: HOXD11 directly bounded with the promoter of FN1 in PSCC. | Cell Death & Disease

Fig. 5: HOXD11 directly bounded with the promoter of FN1 in PSCC.

From: miR-138-5p-mediated HOXD11 promotes cell invasion and metastasis by activating the FN1/MMP2/MMP9 pathway and predicts poor prognosis in penile squamous cell carcinoma

Fig. 5

A GO enrichment analyses between Penl2 HOXD11-shNC and HOXD11-sh1 cells indicated the significant genes enrichment involving in tumor progression. B The differential genes involving in EMT and cell adhesion are shown by heatmap. C GSEA analysis showed that knockdown of HOXD11 might promote EMT and tumor metastasis through the degradation of ECM. D qPCR analysis of ECM-related markers in the indicated cells. E Luciferase reporter assay of FN1 and THBS1 transcriptional activity. F Six potential FN1 promoter binding regions with HOXD11 were predicted by JASPAR database. The chromatin stabilization of corresponding regions were detected by H3K27Ac, H3K4Me1 and H3K4Me3 by UCSC Genome Browser. G Detail nucleotide binding sequences of FN1 promoter and the CHIP-seq results. H, I Luciferase reporter and CHIP-seq assays indicated that FN1 transcriptional activity was eliminated when transfection with FN1 promoter with mutant nucleotide binding sequences of P2 sites. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. KEGG, Kyoto Encyclopedia of Genes and Genomes; GO, Gene Ontology; GSEA, gene set enrichment analysis; EMT, epithelial-mesenchymal transition; ECM, extracellular matrix; CHIP, chromatin immunoprecipitation.

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