Fig. 4: Polydesaturation causes ER stress-related apoptosis and modifies cell cycle in TSCs. | Cell Death & Disease

Fig. 4: Polydesaturation causes ER stress-related apoptosis and modifies cell cycle in TSCs.

From: Lipid desaturation regulates the balance between self-renewal and differentiation in mouse blastocyst-derived stem cells

Fig. 4

TSCs (129/black 6) were treated by SC-26196 (0.2 µM) or DMSO control for 48 h in feeder free culture. Cell counting was carried out using CytoSmart cell counter and validated by manual counting (A; n = 4). Colony numbers per field were estimated under the microscope (B; 5 fields taken for each well, for 4 wells). The expression of IRE1, ATF6 and ASP was examined by RT-qPCR following D6D inhibition for 24 h (C; n = 4). Apoptosis was assessed in TSCs following D6D inhibition for 24 h using Annexin V/PI staining (D, E; n = 4). The numbers in each quadrant of dot plot represent the percentage of cells. Cell cycle was analyzed following inhibition of D6D by SC-26196 for 24 h. The numbers in each quadrant of dot plot represent the percentage of cells (F, G; n = 4). Data are presented as mean ± SEM. *P < 0.05; ***P < 0.001.

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