Fig. 2: Pyroptosis, apoptosis, and necroptosis were triggered simultaneously in UVB-challenged mice. | Cell Death & Disease

Fig. 2: Pyroptosis, apoptosis, and necroptosis were triggered simultaneously in UVB-challenged mice.

From: GSDME deficiency leads to the aggravation of UVB-induced skin inflammation through enhancing recruitment and activation of neutrophils

Fig. 2: Pyroptosis, apoptosis, and necroptosis were triggered simultaneously in UVB-challenged mice.

A The level of keratinocyte death was determined by PI staining. Wide spectrum Cytokeratin was used to indicate keratinocytes. Scale bar represents 100 μm, n = 5. B, C Caspase 3 (B), and phosphorylation at Ser345 site of MLKL (C) were detected by immunofluorescence assay. The nucleus was stained by Hoechst (n = 5). Scale bar represents 100 μm. D PARP, caspase 3, and phosphorylation at Ser345 site of MLKL were detected by western blotting assay in WT or Gsdme−/− mice at 24, 48, and 72 h after UVB exposure. Quantification of the interested protein bands was shown (n = 3). *P < 0.05. NS nonsense.

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