Fig. 1: Hepatic-specific p66Shc knockdown protects against oxidative stress, apoptosis and inflammation in liver I/R.

A The protein expression of p66Shc in the livers of normal human and patients undergoing liver transplantation, n = 6. B The protein expression of p66Shc in the livers of mice exposed to 1 h of ischemia followed by 0, 1, 3, 6, and 12 h of reperfusion, n = 3. C Immunohistochemistry staining for p66Shc in the livers of mice exposed to 1 h of ischemia followed by 6 h of reperfusion. Scale bar, 100 μm. D–J AAV-TBG-p66Shc shRNA was injected to mice followed by liver I/R. D p66Shc mRNA (n = 6) and p66Shc protein expression (n = 3). E H&E staining. Scale bar, 100 μm. F Serum ALT, AST and LDH levels, n = 8. G Liver IL-1β, IL-6 and TNF-α mRNA levels, n = 6. H Liver CAT, SOD, H₂O₂ and MDA contents, n = 8. I TUNEL staining. Scale bar, 100 μm. J p66Shc, Bcl2, Bax and, C cas-3 protein expression, n = 3. ^#P < 0.05, ^##P < 0.01.