Fig. 5: USP15 knockdown protects against oxidative stress, apoptosis, and inflammation in liver I/R.
From: USP15 regulates p66Shc stability associated with Drp1 activation in liver ischemia/reperfusion
A The protein expression of USP15 in the livers of normal human and patients undergoing liver transplantation, n = 6. (B) USP15 protein expression in the livers of mice exposed to 1 h of ischemia followed by 0, 1, 3, 6, and 12 h of reperfusion, n = 3. C USP15 protein expression in AML12 cells subjected to 12 h of hypoxia followed by 0, 1, 2, 4, and 8 h of reoxygenation. D–J AAV-TBG-USP15 shRNA was injected into mice followed by liver I/R. D USP15 mRNA expression, n = 6. E H&E staining. Scale bar, 100 μm. F Serum ALT, AST and LDH levels, n = 8. G Liver IL-1β, IL-6 and TNF-α mRNA levels, n = 6. H Liver CAT, SOD, H2O2, and MDA contents, n = 8. I TUNEL staining. Scale bar, 100 μm. J USP15, p66Shc, Bcl2, Bax, and C-cas-3 protein expression. K AML12 cells were transfected with USP15 siRNA under H/R conditions. USP15, p66Shc, Bcl2, Bax and C-cas-3 protein expression, n = 3. #P < 0.05, ##P < 0.01.
