Fig. 1: The methylation profile of the HOXA5 gene in ECCA.

A Heatmap analysis of the methylation and expression level of the HOX genes. The gradual changes from red to white represent changes in gene expression from high to low. Green, no CpG island. Blue, 1 CpG island. Red, 2 CpG islands. B, C RT-qPCR and Western blot analyses of HOXA5 expression in ECCA cells following treatment with, or without, DCA. D, E Analysis of the methylation profile of the HOXA5 gene in GSE49656 and GSE38860 of GEO database. The purple box represents the cancer samples and the blue box represents the non-tumor samples. F Bubbles plotted the KEGG analysis of the methylation levels of differential genes. The gradual changes from red to blue represent the changes in high to low FDR. The sizes of bubbles represent the numbers of genes enriched in certain terms. G The methylation profile of the HOXA5 gene from the BSP results. The black solid represents methylated sites and the hollow points represent unmethylated sites. *P < 0.05. **P < 0.01. ***P < 0.001.