Fig. 4: Identification of MXD1 as the potential target of HOXA5. | Cell Death & Disease

Fig. 4: Identification of MXD1 as the potential target of HOXA5.

From: HOXA5 inhibits the proliferation of extrahepatic cholangiocarcinoma cells by enhancing MXD1 expression and activating the p53 pathway

Fig. 4: Identification of MXD1 as the potential target of HOXA5.The alternative text for this image may have been generated using AI.

A Heatmap analysis of the top DEGs from RNA-seq analysis of HOXA5 overexpression in ECCA cells. The gradual changes from blue to red indicate the expression levels from low to high. B, C Bubble plotted for the DEGs and TFBS enriched in the different pathways. The gradual changes from red to blue indicate in FDR from high to low. The sizes of bubbles represent the numbers of genes enriched in certain terms. D Venn diagram displayed the intersection between terms of TFBS and the DEGs. E Venn diagram exhibited the intersection between the predicted target gene of MYC-MAX and E2F terms. F Validation of E2F2, E2F7 and E2F8 expression levels by RT-qPCR in HOXA5 overexpressed cells. G, H Validation of MYC and MXD1 expression levels by RT-qPCR and Western blot in HOXA5 overexpressed cells. I Immunohistochemistry analysis of MXD1 expression in the tumors from nude mice. *P < 0.05. **P < 0.01. Orange bar, 50 μm. Green bar, 20 μm.

Back to article page