Fig. 7: The HOXA5/MXD1 axis enhances p53 expression and HOXA5 directly induces MXD1 transcription in ECCA cells. | Cell Death & Disease

Fig. 7: The HOXA5/MXD1 axis enhances p53 expression and HOXA5 directly induces MXD1 transcription in ECCA cells.

From: HOXA5 inhibits the proliferation of extrahepatic cholangiocarcinoma cells by enhancing MXD1 expression and activating the p53 pathway

Fig. 7: The HOXA5/MXD1 axis enhances p53 expression and HOXA5 directly induces MXD1 transcription in ECCA cells.The alternative text for this image may have been generated using AI.

A–C HOXA5 or MXD1 overexpression increased p53 protein expression, and the enhanced effect of HOXA5 overexpression on p53 protein expression was mitigated by MXD1 silencing in ECCA cells. D Validation of the target genes involved in the p53 pathway. E The predicted binding sequences of HOXA5 in the MXD1 promoter from the JASPAR database. F The predicted binding sites in the MXD1 promoter from the JASPAR database. G The sequences of four sites and the scheme of the mutations in this study. H, I Luciferase reporter assays indicated that HOXA5 expression enhanced the MXD1 promoter-controlled luciferase expression, which was abrogated by the site C mutation in the MXD1 promoter in HEK293T cells. J ChIP validated that HOXA5 expression increased its binding to the MXD1 promoter at the predicted site, which was inhibited by the mutation in the DNA binding domain of HOXA5. *P < 0.05. **P < 0.01. ***P < 0.001.

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